Coding

Part:BBa_K4362061:Design

Designed by: Xuyang Ran   Group: iGEM22_Tianjin   (2022-09-08)


to gain kanamycin resistance


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 178
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 178
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 178
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 178
    Illegal NgoMIV site found at 629
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 478
    Illegal SapI.rc site found at 688


Design Notes

none


Source

Cloning vector pEASY-tub/neo, complete sequence Sequence ID: MN481464.1Length: 6580

References